Troubleshooting Chromatographic
Systems
Chromatographic instruments are an integral part
of almost every analytical laboratory. While modern instruments are very
reliable, chromatographers must still deal with occasional problems (peak
shape changes, baseline shifts, retention time problems, etc.) that can
arise from the instrument, the sample, or the laboratory. This seminar
will provide guidance on identifying the causes of such problems, finding
solutions, and preventing future problems. Basic LC and GC components
will be discussed, and helpful hints will be provided on how to avoid
certain problems and maximize the overall analytical efficiency in the
laboratory. Come prepared to discuss your own situations as part of an
interactive discussion.
Students will learn about:
- General troubleshooting strategies
- The importance of evaluation mixtures
- Common symptoms
- Common solutions to common symptoms
Outline
- General Troubleshooting Strategies
- Isolating the Problem
- Common symptoms
- Using Evaluation Mixtures
- Troubleshooting Gas Chromatography Systems
- GCs Today
- Autosamplers and Inlets
- Capillary Columns
- Detectors
- Plumbing
- Ancillary Techniques
- Prevention Strategies
- If All Else Fails
- Troubleshooting Liquid Chromatography Systems
- System Components
- Design Characteristics
- Potential Problems
- Symptoms and Solutions
- Data Systems, Peak Integration and Reporting
Issues
- When should you integrate with peak height
rather than peak area?
Course Length: 1 or 2 days
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