Best
Practices in HPLC
Who
Should Attend?
- HPLC operators and analyst
- Users who want to upgrade their operationg practices to improve efficiency and reliability
- Supervisory
staff
|
Ever wonder why some labs seem to operate well with few problems, while others are always struggling to keep the equipment working? It's all about "best practices" in the lab. There are things you should do and things you should not do! There are things that help and things that don't! This presentation will help you sort out the difference.
Topics:
- Getting Started
- Those Important First Questions
- The HPLC Universe
- Care and Maintenance of Your System
- Operating Practices
- Don’t Forget About the Sample
- Settings
- Equilibration
- Startup and Shutdown
- Data Analysis
- Calculations
- Reviewing Chromatograms
- Integration Best Practices
|
Best
Practices in HPLC Column Selection
Who
Should Attend?
- Method development
staff
- Researchers
who want to better understand column selectivity
- Supervisory
staff
|
- Confused about which column to
use for your HPLC method?
- Not sure why a particular column
was chosed?
- Want to find an equivalent column,
or a better column, or a different column?
The C18 column is the most popular
option for HPLC methods, but is it the best choice for your method?
There are many other options, and we will help you understand why
some columns work well while others do not. This presentation uses
our own research along with publicly available resources to help
you understand how to begin the column selection process.
Topics:
- What is selectivity?
- Reversed phase vs. HILIC vs. mixed
mode
- Measuring selectivity
- Selectivity resources and databases
- Examples
|
HPLC
Basics for Quality Staff and Auditors
Who
should attend?
This seminar is for auditors, other
Quality staff, and supervisors who do not have a technical background
in chromatography, but must review and approve chromatographic data.
|
Chromatographic data
are used to answer critical questions - drug purity, content uniformity,
impurity levels, etc. The language and practices of HPLC are complex
and not always completely understood, even by users. Review of these
data sets often is performed by auditors who do not have a laboratory
background in these techniques and discussing the results with chromatographers
can be difficult if you don't speak the "language of chromatography."
This presentation provides an introduction
to HPLC with an emphasis on understanding the language and practices
that an auditor needs to properly review chromatographic data. Topics
will include a discussion of the components and how their failure
can affect results, a review of common chromatographic problems,
and suggestions for dealing with problems.
Topics:
- Brief introduction to chromatography
and HPLC
- Brief description of HPLC components
and their associated problems
- The language of chromatography
- Checklist for reviewing data
- Basic troubleshooting - what
happens when a component fails
- Investigating problem data
- Real-world examples
|
Reducing
Integration Errors In Chromatography - Part 1: When Peaks Are Approximately
the Same Size
Who
Should Attend?
-
All
chromatographers can benefit from this presentation.
- Reviewers
will also find the content to be useful.
|
Integration of chromatographic peaks
is a critical step in the generation of chromatographic data. Errors
in integration will result in a corresponding error in calculated
results. Unfortunately, most analysts have little or no training
in the proper procedures for peak integration. We will first discuss,
in general terms, how integrators work. Then, peak integration issues
will be examined using sets of real chromatographic data to illustrate
when to use different integration strategies (drop, valley, etc.).
We will discuss a variety of situations involving poorly resolved
chromatographic peaks, when the peaks are about the same size.
Topics:
- Introduction to chromatographic
integrators
- How do they find the peaks?
- What does resolution mean when
the peaks are not of equal size?
- What integration baseline options
are available?
- Integration errors for each baseline
option and recommendations for selecting the best option.
- Sources of integration errors
- Why are the numbers wrong?
- Discussion - Questions and Answer
|
Reducing
Integration Errors In Chromatography - Part 2: Impurity and Trace
Analysis Situations
Who
Should Attend?
- Chromatographers
who use trace analysis or impurity methods will find this information
to be useful.
- Reviewers
will also benefit from the content.
|
The measurement of small peaks is
a particularly challenging analytical problem, especially if there
is a large peak next to the small peak. This webinar will focus
on the integration errors that can result from the use of the wrong
integration method. The erros can be large (more than 100%). Attend
this seminar to learn the best way to integrate small peaks.
Topics:
- What integration baseline options
are available?
- Review of results when peaks are
of approximately equal size.
- Integration errors for each baseline
option and recommendations for selecting the best option.
- Sources of integration errors
for small peaks.
- More complex topics
- Tailing, unresolved peaks
- Discussion - Questions and Answers
|
Understanding
the Calibration Curve
Who
should attend?
- Analysts who must generate and
use calibration curves.
- Method development staff who much
design the calibration system for methods.
- Auditors and supervisory staff
who must review or troubleshoot analytical data
|
Almost every analytical method requires
some form of "calibration," but how do you know if your
calibration is "good?" In this webinar you will learn
the options for setting up calibration curves, whether you use a
single standard or several different levels. You will also learn
how to evaluate your calibration data, and find potential problems
before they ruin your data. Based on our half day presentation of
the same name, this webinar has unique practical content that is
not available from any other source.
Topics:
- Calibration Basics
- Types of Calibrations
- Selecting Calibration Levels
- Calibration Options
- Evaluation of Data
- Why the Correlation Coefficient
Is Not Always a Good Indicator of Calibration Quality
- Real World Data Sets (GC, LC,
AA, GC-MS, etc.)
|
Practical
Laboratory Statistics
Who
should attend?
- Analysts who must generate and
use analytical data.
- Auditors who review reports
- Supervisory staff who must review
or troubleshoot analytical data
|
This webinars offers a practical discussion of statistical principles, with very little statistical
"theory". We look at the most common statistical techniques
that are used in evaluating analytical chemistry data, such as:
- How to calculate means, standard
deviations, and confidence intervals
- How to properly reject data points
- How to spot trends in your data
- How to determine if two standard
deviations are different from each other (comparing precision)
- How to determine if two means
are different from each other (comparing accuracy)
These topics are taught by working
through real analytical chemistry examples. Based on our half day
presentation of the same name, this webinar offers a rapid way to
learn the terminology of statistics.
|
Introduction
to Cirrus GPC Software |
The Cirrus GPC software is a popular
option for doing GPC analysis. It has recently been updated for
use with OpenLab/ChemStation software. This webinar will provide
an overview of the important Cirrus settings, allowing you to integrate
with your ChemStation data acquisition.
Topics:
- The Workbook Concept
- Setting up Your First Workbook
- GPC Settings
- Setting up Your First Calibration
Curve
- Analyzing Samples
|
Instructor |
Merlin K. L. Bicking,
Ph.D. is President, ACCTA, Inc. He has been actively involved in chromatography
for more than 25 years, and has been teaching ChemStation users for
10 years. The topics in these seminars are drawn from existing on-site
and on-line classes, and his own experience in the laboratory. |