Webinar Topics

Best Practices in HPLC

Who Should Attend?

  • HPLC operators and analyst
  • Users who want to upgrade their operationg practices to improve efficiency and reliability
  • Supervisory staff


Ever wonder why some labs seem to operate well with few problems, while others are always struggling to keep the equipment working? It's all about "best practices" in the lab. There are things you should do and things you should not do! There are things that help and things that don't! This presentation will help you sort out the difference.


  • Getting Started
    • Those Important First Questions
  • The HPLC Universe
    • Care and Maintenance of Your System
  • Operating Practices
    • Don’t Forget About the Sample
    • Settings
    • Equilibration
    • Startup and Shutdown
  • Data Analysis
    • Calculations
    • Reviewing Chromatograms
    • Integration Best Practices

Best Practices in HPLC Column Selection

Who Should Attend?

  • Method development staff
  • Researchers who want to better understand column selectivity
  • Supervisory staff


  • Confused about which column to use for your HPLC method?
  • Not sure why a particular column was chosed?
  • Want to find an equivalent column, or a better column, or a different column?

The C18 column is the most popular option for HPLC methods, but is it the best choice for your method? There are many other options, and we will help you understand why some columns work well while others do not. This presentation uses our own research along with publicly available resources to help you understand how to begin the column selection process.


  • What is selectivity?
  • Reversed phase vs. HILIC vs. mixed mode
  • Measuring selectivity
  • Selectivity resources and databases
  • Examples



HPLC Basics for Quality Staff and Auditors

Who should attend?

This seminar is for auditors, other Quality staff, and supervisors who do not have a technical background in chromatography, but must review and approve chromatographic data.


Chromatographic data are used to answer critical questions - drug purity, content uniformity, impurity levels, etc. The language and practices of HPLC are complex and not always completely understood, even by users. Review of these data sets often is performed by auditors who do not have a laboratory background in these techniques and discussing the results with chromatographers can be difficult if you don't speak the "language of chromatography."

This presentation provides an introduction to HPLC with an emphasis on understanding the language and practices that an auditor needs to properly review chromatographic data. Topics will include a discussion of the components and how their failure can affect results, a review of common chromatographic problems, and suggestions for dealing with problems.


  • Brief introduction to chromatography and HPLC
  • Brief description of HPLC components and their associated problems
  • The language of chromatography
  • Checklist for reviewing data
  • Basic troubleshooting - what happens when a component fails
  • Investigating problem data
  • Real-world examples


Reducing Integration Errors In Chromatography - Part 1: When Peaks Are Approximately the Same Size

Who Should Attend?

  • All chromatographers can benefit from this presentation.
  • Reviewers will also find the content to be useful.


Integration of chromatographic peaks is a critical step in the generation of chromatographic data. Errors in integration will result in a corresponding error in calculated results. Unfortunately, most analysts have little or no training in the proper procedures for peak integration. We will first discuss, in general terms, how integrators work. Then, peak integration issues will be examined using sets of real chromatographic data to illustrate when to use different integration strategies (drop, valley, etc.). We will discuss a variety of situations involving poorly resolved chromatographic peaks, when the peaks are about the same size.


  • Introduction to chromatographic integrators
    • How do they find the peaks?
  • What does resolution mean when the peaks are not of equal size?
  • What integration baseline options are available?
  • Integration errors for each baseline option and recommendations for selecting the best option.
  • Sources of integration errors
    • Why are the numbers wrong?
  • Discussion - Questions and Answer


Reducing Integration Errors In Chromatography - Part 2: Impurity and Trace Analysis Situations

Who Should Attend?

  • Chromatographers who use trace analysis or impurity methods will find this information to be useful.
  • Reviewers will also benefit from the content.


The measurement of small peaks is a particularly challenging analytical problem, especially if there is a large peak next to the small peak. This webinar will focus on the integration errors that can result from the use of the wrong integration method. The erros can be large (more than 100%). Attend this seminar to learn the best way to integrate small peaks.


  • What integration baseline options are available?
  • Review of results when peaks are of approximately equal size.
  • Integration errors for each baseline option and recommendations for selecting the best option.
  • Sources of integration errors for small peaks.
  • More complex topics
    • Tailing, unresolved peaks
  • Discussion - Questions and Answers


Understanding the Calibration Curve

Who should attend?

  • Analysts who must generate and use calibration curves.
  • Method development staff who much design the calibration system for methods.
  • Auditors and supervisory staff who must review or troubleshoot analytical data


Almost every analytical method requires some form of "calibration," but how do you know if your calibration is "good?" In this webinar you will learn the options for setting up calibration curves, whether you use a single standard or several different levels. You will also learn how to evaluate your calibration data, and find potential problems before they ruin your data. Based on our half day presentation of the same name, this webinar has unique practical content that is not available from any other source.


  • Calibration Basics
  • Types of Calibrations
  • Selecting Calibration Levels
  • Calibration Options
  • Evaluation of Data
  • Why the Correlation Coefficient Is Not Always a Good Indicator of Calibration Quality
  • Real World Data Sets (GC, LC, AA, GC-MS, etc.)


Practical Laboratory Statistics

Who should attend?

  • Analysts who must generate and use analytical data.
  • Auditors who review reports
  • Supervisory staff who must review or troubleshoot analytical data


This webinars offers a practical discussion of statistical principles, with very little statistical "theory". We look at the most common statistical techniques that are used in evaluating analytical chemistry data, such as:

  • How to calculate means, standard deviations, and confidence intervals
  • How to properly reject data points
  • How to spot trends in your data
  • How to determine if two standard deviations are different from each other (comparing precision)
  • How to determine if two means are different from each other (comparing accuracy)

These topics are taught by working through real analytical chemistry examples. Based on our half day presentation of the same name, this webinar offers a rapid way to learn the terminology of statistics.


Introduction to Cirrus GPC Software

The Cirrus GPC software is a popular option for doing GPC analysis. It has recently been updated for use with OpenLab/ChemStation software. This webinar will provide an overview of the important Cirrus settings, allowing you to integrate with your ChemStation data acquisition.


  • The Workbook Concept
  • Setting up Your First Workbook
  • GPC Settings
  • Setting up Your First Calibration Curve
  • Analyzing Samples


Merlin K. L. Bicking, Ph.D. is President, ACCTA, Inc. He has been actively involved in chromatography for more than 25 years, and has been teaching ChemStation users for 10 years. The topics in these seminars are drawn from existing on-site and on-line classes, and his own experience in the laboratory.